suzuki gsx r 1000

Modif Terbaru Suzuki Satria FU 150 Style

Suzuki Satria FU 150 Style

Although we intip ahead, but who knows if in fact there are several new features looks behind the New Satria F150. "There are some features that differ," Edi bisik found in the middle ramainya JMS 2008.Dari on let's open it's the latest detail duck with engine capacity of at most this.

Most obvious change would have on the design of the lamp cover. Head lamp design and covernya become the main attraction. Suzuki already has from the first characteristic of MOGE always provide a touch of duck including the motor for this latest Satria F150. Head lamp Suzuki GSX 600R entered to try to slim the body Suzuki F150.

Suzuki Satria FU 150 Style

Great! it is the right word given to the Suzuki Satria F-150 this. Imagine, Topo Goedhel Atmodjo's Custom Tauco (TC), so changing the shape of a duck sport model with the body part is minimal. "With the body part that can also show little ability to play detail," said Topo.

As the order of Tubular iron pipe, for example. Usually, in the middle part is left empty, but closed by Topo use triangular plate. Not easy work because the closed must remember the precise contours of each field have. In fact, do so with perfect Topo.

2010 THUNDER 250 CC PICS MODIFIED SPECS

2010 Suzuki Thunder 250 specification modifications :

KALIPER front: Brembo 4 piston Brembo master
KALIPER back: Nissin 2 piston Nissin brake master
Front disc: 300 mm
Cakarm back: 240 mm
Sok fronts: GSXR400 Showa
Sok back: Showa
Swing-arm: Pro-arm VFR 400
Footstep: Copy of the Honda CBR400
Front rim: Enkei 3:50 x17
Front tires: Pireli 120/60-17
Rear rim: Enkei 4:50 x18
Rear tires: Pireli 160/60-17
Handlebar: Kawasaki Ninja, Yamaha FZR Raiser
Closed tank: Kawak Ninja
Deltaboks: Fiberglass
Fairing: Acerbis
Speedometer: Suzuki Bandit
Headlights: Acerbis
Windshield: Acerbis
Body rear: JMS Design
Stirrup back: Suzuki Thunder
Taillights: Honda CBR150
Hood: JMS Design

headlamp suzuki thunder 250 cc

rear rims of suzuki thunder 2

Suzuki Gladius Concept by Nasty GPDESIGN

GPDESIGN proposes an interesting concept motorcycle called Nasty based Suzuki Gladius 650. The concept of the motion is focused on technology and innovation leading to customize both design and emphasize safety. Concept Nasty has the task of entertaining the motorcyclist in everyday life, with versatility.

The saddle height from the ground has been increased by 70 mm and increased to 855 mm. The range was adjusted to allow a more sporty. The surface of the saddle was made with high quality water resistant leather, the upholstery has been increased to 2 cm. The double stem section 22-28 has completed a new curvature from raiser and coverage between the two plates look more impressive. In the version submitted for the painting was used an innovative material that fruit properties of photo luminescence, this type of material was used for the cuffs, the tank and wheels. The property of the photo luminescence is to accumulate a certain quantity of both natural and artificial light and then return the shine in the dark for a period of about 4-5 hours. Were used and exhaust tip from the official catalog SUZUKI. Nasty will continually evolving and customized as desired by allowing the end user to have a motorcycle customized.

New Satria FU Modif Simple Design

Today, he wants to hunting cute girls with Satria FU simple modif. What else if not the modif motors that must also dress up cute! "Said M. Nursalim, SMADA students who stay in the area Pulungan Pandaan.

Doi necessarily then dressed her Suzi by Satria FU-nge-style race style emang again booming community of slang among Pandaan. Penggarapannya fully completed by Fertile, big boss emang Thole Concept which have special Specializing in daily use MODIF design style.

The figure's legs were closed immediately cast wheelnya, then contrived settingannya spoke wheel with R eks. Satria Tromol support for the stern and Shogi 125 for the front. "The selection of ex Tromol Suzuki products more mengampangkan coz we have the same mounting bracket New Satria F Modif both rear gear and bushing-bushingnya" Fertile straightforward.

Reaffirming accent, racing wheel racing wheel follow-up dicangkoki almu U-Shape profile of the tire circumference mated cungkring 215-17. Moreover, direct digencarkan finishing projects with a total whitewash frame with metallic green color which more and more candy aura racing.

Body setnya white stained rehearsal had additive effect with shades of pearl yellow flag start. Increasingly small and attractive appearance when keresikan backed up his body-color and luster chrome titan in some sudutnya.tito

Specification of New Satria Fu MODIF
Rim: TDR, BAN: Mizzle-Swallow, Handle: Ride It, GAS: Bungbon, Grip: Moto GP, Coil: Ride It, FOOT STEP: Posh, CAT: Lumina White Gold, chromium: Chromium Bangil AB, modifier: Thole Concept , Jl.Ry Pandaan Cow Statue, Rear Yan Motor by Fertile.

MODIF SUZUKI SHOGUN 125 SP PICS COLLECTION

suzuki shogun 125 sp modification under this I get a pretty hard. because suzuki shogun 125 sp is still a bit of the modification and the spread on the internet. perhaps modification suzuki shogun's image is similar to other weblogs that have made the posting about the modifications suzuki shogun 125 sp. not what's important I also have a posting that contains the modifications suzuki shogun 125 sp. I have three pictures MODIF suzuki shogun 125 sp which is roughly the image when the motor modification in the title contest. The best picture is the number three. because I see a modification of the bike is very unique and beautiful clean look to the eye and have a high selling value. because the price of the modification of the motor is more expensive than the original motor from the factory.

suzuki shogun 125 sp modif pics 1



pics 2 modifikasi suzuki shogun 125 sp



pics 3 of modif suzuki shogun 125 sp

Suzuki GSX 750 Limited version

England - Suzuki GSX-R750 launch GB Limited Edition, by preparing as many as 25 units. Lovers of big motor this motor can make a reservation exclusively through www.imgsx site-r.com.

In the event the International Motorcycle & Scooter Suzuki, Suzuki GSX-R750 Limited Edition, managed to make many people amazed after seeing the performance of the firm and large bodynya like a Superbike racing motorcycle.

David Taylor was director of sales and marketing of Suzuki UK, said, "The limited production, because we want this to be a motor bike that comes with significant momentum. Because the amount of production a little, GSX-R750 into the motor that has a charm that can not be paid for with money. So that at any price and keep customers willing to pay. In the end it became a motor bike if it is owned pride. "

Features of this motor has a unique color composition using paint retro, this is the result of a change from the GSX-R750 has ever issued in the year 1996. Appearances pillars with the best body design, this motor show is very tough. Plus the quality of artificial Yoshimura exhaust directly. (Elly)

Hayabusa 1300/gsx1300r Complete Specification in Selling

Hayabusa 1300/gsx1300r Complete Specification in selling motor good condition, used a new month.

Specification:
Engine: 4-cylinder 2-tak, liquid-cooled, DOHC ,4-valve
Capacity: 1340cc
Bbm supply system: Fuel injection
Ignition: fullytransistorised
Ignition system: Electrik
Tranmisi: 6-speed constan mesh)
Dimensions (PXLXT): 2195 x 740 x 1170 mm

New Satria FU Modif Simple Design

Today, he wants to hunting cute girls with Satria FU simple modif. What else if not the modif motors that must also dress up cute! "Said M. Nursalim, SMADA students who stay in the area Pulungan Pandaan.

Doi necessarily then dressed her Suzi by Satria FU-nge-style race style emang again booming community of slang among Pandaan. Penggarapannya fully completed by Fertile, big boss emang Thole Concept which have special Specializing in daily use MODIF design style.

The figure's legs were closed immediately cast wheelnya, then contrived settingannya spoke wheel with R eks. Satria Tromol support for the stern and Shogi 125 for the front. "The selection of ex Tromol Suzuki products more mengampangkan coz we have the same mounting bracket New Satria F Modif both rear gear and bushing-bushingnya" Fertile straightforward.

Reaffirming accent, racing wheel racing wheel follow-up dicangkoki almu U-Shape profile of the tire circumference mated cungkring 215-17. Moreover, direct digencarkan finishing projects with a total whitewash frame with metallic green color which more and more candy aura racing.

Body setnya white stained rehearsal had additive effect with shades of pearl yellow flag start. Increasingly small and attractive appearance when keresikan backed up his body-color and luster chrome titan in some sudutnya.tito

Specification of New Satria Fu MODIF
Rim: TDR, BAN: Mizzle-Swallow, Handle: Ride It, GAS: Bungbon, Grip: Moto GP, Coil: Ride It, FOOT STEP: Posh, CAT: Lumina White Gold, chromium: Chromium Bangil AB, modifier: Thole Concept , Jl.Ry Pandaan Cow Statue, Rear Yan Motor by Fertile.

Satria FU Modif 2009 Sidoarjo - Positive Rebellion

Satria FU Modif 2009 Sidoarjo-Parents could forbid modifying the motor, but Faruch've already dead in love with customizing motorcycles so reckless violation of the ban doi parent. "What's important I remain respectful and not brash with parent and I'm not a drug," he argued.

Junior high school students this Sidoarjo go to five outlets will close Budhienk Modified racing style trends. After consultation with the chosen hue modifier, the results look funky racing.

Bodi appeared compelling after modification mengguyurnya Budi padepokan owners with paint galaxy. It seems unimpressed plain varnish also pointed galaxy.
Sector appear attractive legs after sleeve pretentious front, rear and standard motor Footstep washed down by AB CHROME chrome layer Bangil.

Not to forget aseso legs also installed the U-shape rim TDR model clad in front of the rear tires Duro. cand

Spesification Satria FU MODIF :
Rim: TDR, BAN: Duro 60/80-17, SOK DPN: chrome, vinyl: Ride It, Tromol DPN: trusty, Tromol BLKG: Variations, CAT: Galaxies, varnish: Galaxies, modifier: Modified Budhienk, Tenggulunan Road, Sidoarjo tel. 085 730 700 001.

Suzuki Shogun 125 Extreme

The aboriginal Suzuki SHOGUN 125 assembly in 2006.
Data modif:
Tire front: Battlax 120/60-17
Rear tire: Battlax 150/60-14
Pelek: Suzuki GSX400
Upside down: Suzuki GSX400
Handlebar: Suzuki GSX400
Swing-arm: Aprilia 125
Sok back: Aprilia 125
Rem: Suzuki GSX400
Body: Custom
Radiator: Jupiter MX 135LC
Spidometer: Satria F-150
Footstep: Aprilia 250
While the additional SHOGUN 2005.
Bodi dikonsep cilia architecture with the monster characters. Seabrek uređaja audio added. Result, this motor dinobatkan pants as a artery monster.
Modif Data:
Ban: Mizzle 50/90-17
Velg: Yoshi
Cakram: 4 units TZM
Shock dpn: Up Side Down
Shock Blk: Kitaco
Arm swing: Posh
Exhaust: Costum 4 hole
Cat: Blue Graphic Gliter
Body: Monster Costum
Head Unit: avt
Power: Symbian
Monitor: Liliput
Sub woover, Midle & Twiter: Venom.

Suzuki Spin 2010 Modifikasi

Suzuki Spin 2010 Modifikasi, The aspect of point and the absolute rumours consistently heating were the architect the 'weapon able of the motor cartage of S of the apple to put on jack to the top the mark they. Proven in anniversary acknowledgment of the motor cartage of the world, the bike of abstraction of war which was abounding by approaching technology favorable to the environment, consistently emerged. But with attention which success was the bags of penarik of allurement of visitors. As in the armpit of the 29th all-embracing agent of Bangkok (BIMS) appearance in the Na architecture of draft of BITEC, Bangkok, Thailand (27/3-6/4).

Modifikasi Suzuki Shogun 125

- Izumi agent 54 jenong 6 mm
- Control agent crankshaft afterward jupiter z
- 27/23 valve
- Carburettor pwl26 aboriginal kawasaki ninja
- The arrangement of absolute manual change is
- Absolute accident ignition
- CDI ability cheetah
- Coil 4ss
- Oil bolt tank, billow tank
- Dpn velg 160x17 rear tk
- Tires federalr
- Daytona rear shock, accepted advanced jupiter z
- Accepted array aboriginal Vega
- Speedometer by koso
- Chain + overdrive set by Daytona 415H
- Fuel: admixture of benzene by a + kl lg roadrace, + added carapace mixture.

Modifikasi Suzuki Motor 500 cc

Suzuki Brigand 500cc Sport Bike Modification Specifications Detail:
Velg advanced Rim of the aboriginal 50cc suzuki bandit
Velg aback Rim of the aboriginal 50cc suzuki bandit
Body aboriginal suzuki brigand motor 50cc
Original beat arm brigand suzuki 50cc
suzuki brigand bankrupt 50cc
shock advanced is still application the aboriginal shock telescope
the framework of the aboriginal 50cc suzuki bandit
under the aboriginal allowance suzuki brigand 50cc
front annoy admeasurement 110/80 Swallow Deli Tire
rear annoy admeasurement 130/80 Swallow Deli Annoy

2003 Suzuki Raider Extreme Modification

No more powerful features of the 2003 Suzuki Raider. Starting from the front to rear, this bike has changed completely. Instead the result so extreme, with some elements of the technology changes. Like condoms tank lid can be opened using keys, that one is a breakthrough. At the very least, a breakthrough modifications in Pontianak, West Borneo.

Home modifications Irfandi from Pontianak in 2XP "Equator City" made a bold move. Consider the attached seat on the house from the original exhaust Ducati 969. Meanwhile, the bottom-distance with the wheels still empty. "Indeed, the design was not able to fit," said Irfandi.

Plus the arm (swing arm) are large and sturdy, while the framework for and the seat is thin, the harmonization was a little disturbed. "In the arm, I use the pro arm of Honda NSR SP and condoms to be given greater," explained Irfandi.

To the top, using a subframe which modificator is knock down because he wanted to make a long tail. If you get bored, then it could be dismantled extra-pair. To integrate additional context, he uses the L bolt size of 8 mm 6 pieces. "In addition to booster seats, it also became a kind of bracket for the exhaust," said senior modifikator.

Realized there was a wide cavity, he cut back by adding exhaust Ducati 969. Another thing if a minimoto shock breaker position in Australia was not too collapsed. For each, he wore a Honda CBR150 for more tender than the original.

Also interesting, given condoms tank-lid can be opened by pressing the button. According to Irfandi, he uses the power window car fitted with a small shock. By pressing once, the condom could be opened up to 45 degrees.

source: otomotif.kompas.com

Satria F 150cc Modif 2010

SUZUKI SATRIA 150 F EXTREME MODIFICATION
You would not anticipate that a adapted motorcycle belonged to a 55-year-old grandfather. His name Suryana and breeze modif rombakan after-effects are to his satisfaction. Mean, bodies are afterward the actual Jakarta developments and trends modifications.It was so, the abject motor victim Suryana attraction is affectionate of a avoid from Suzuki Satria F-150. To change the "sex" from the avoid into "male model", he handed over the avoid to Tauco Custom (TC) in the Sudirman, South Jakarta, is accepted for authoritative such changes.

SUZUKI SATRIA 150 F EXTREME
full striping modification

suzuki satria fu full striping

Related Posts with thumbnails for bloggerblogger widgets

SUZUKI SATRIA 150 F Rims

Topo Goedhel Atmodjo, skipper TC, annihilate the motor to chase his grandfathering posture. Therefore, the motor was fabricated not so aerial that the accepted Monoshock still in use, including the aboriginal beat arm is alone in-custom bit. Meanwhile, the advanced abeyance upside bottomward archetypal that he fabricated to break adjusted. Gas catchbasin was additionally accountable to accommodate 6 liters. Display motor looks solid because there is a DELTABOX of aqueduct 1 / 2 inches.

satria f 150 cc 2010

Technology FU150 that had engine 147cc, 4 not, DOHC 4 valves, water cooled with SACS (with Suzuki Advanced Cooling System), this that pushed me to buy

SUZUKI SPIN MODIFICATION

Modifications suzuki spin this is the year 2009 with the concept modif x-treme where modifications in the contest, suzuki spin must in the form of modifications have a very good and the jury could draw attention to the modifications in the contest. suzuki spin on the modifications in the form of a very different from the others because matic motor suzuki spin have the form of a unique and different and it is a difficulty in making the modifikator draft modifications suzuki spin them.

Spin Modification Full Airbrush

MODIFIED SPIN COLUMN FOR SIMPLE AND RAPID PLASMID DNA EXTRACTION

Modified Spin Column for Simple and Rapid Plasmid DNA Extraction

Cross-Reference to Related Applications

This application claims priority to United States provisional patent application number 60/941,032 filed 31 May 2007; the disclosure of which is incorporated herein by reference in it entirety.

Field of the Invention

This invention relates to an improved system and method for nucleic acid purification. More specifically, it relates to a simple and rapid system and method for the extraction and purification of plasmid DNA from cells.

Background of the Invention spin

Plasmids are double-stranded supercoiled DNA molecules that range in size from 1 kb to more than 200 kb. Plasmids are useful tools in genetic engineering. They are widely used as vectors to carry foreign DNA; such that the foreign DNA is amplified and isolated or expressed. Plasmid DNA has also been utilized in the development of vaccines and in gene therapy.

The analysis and in vitro manipulation of plasmid DNA is typically preceded by an isolation step in order to free the nucleic acid from unwanted cellular contaminants which may interfere with subsequent processing procedures. A mini-scale sample preparation from an overnight bacterial culture of 1-5 ml generates more than enough plasmid DNA (~ few micrograms) for many of these applications.

The most common plasmid DNA extraction protocols exploit reagents originally developed by Birnboim and DoIy (Birnboim, H. C. and DoIy, J., Nucl. Acids Res. 7, 1513

(1979)), to separate supercoiled plasmid DNA from bacterial genomic DNA, RNA and protein. These reagents, developed many years ago, work on the principle of sequential use of three buffers, commonly referred to as buffer I, II and III. They each have distinct compositions to bring about plasmid enrichment and separation from contaminants. Buffer I is used to resuspend the bacterial pellet obtained by an initial centrifugation step of an appropriate bacterial culture. Once resuspended, buffer II is added which contains SDS detergent and NaOH. These components lyse the bacteria and denature the genomic DNA (pH>12). Buffer III typically contains a chaotrope to further denature protein, the chaotrope also promotes binding of plasmid DNA to the silica matrix commonly used in spin columns. Buffer III also usually contains potassium acetate to rapidly neutralize the combined solutions. The addition of buffer III causes contaminants to "crash-out" of solution owing to the formation of insoluble complexes driven by rapid re-naturation of genomic DNA and the potassium salt of the detergent.

The insoluble flocculant material has traditionally been removed by a centrifugation step to "pack" the flocculant material at the bottom and side of a centrifugation tube (See, e.g. ILLUSTRA™ plasmidPrep Mini Spin Kit, GE Healthcare, Piscataway, New Jersey). The clarified plasmid-containing solution is subjected to a chromatographic separation. For mini-scale purification, the clarified solution is usually applied to a minispin column containing a glass fiber matrix or silica membrane. Plasmid DNA binds to the column in the presence of a chaotrope, while soluble impurities do not bind. After the soluble impurities are washed off, the plasmid DNA are eluted with an appropriate elution buffer.

Recently, alternative buffer compositions have been developed for plasmid DNA extractions based upon 96-well plates, which minimizes the formation of flocculants during bacterial lysis (DIRECTPREP™ 96 Miniprep Kit, Qiagen Inc., Valencia,

California). The use of these buffers generates little precipitated cellular components and eliminates the need to remove the flocculants before column loading of the DIRECTPREP™ 96-well plates. A 96-well plate pre-fϊlter is used to capture any residual precipitants from clogging the silica membrane. However, the columns may become clogged if the cell density is high.

It is advantageous to further simplify the process therefore to provide a more efficient plasmid DNA purification method.

Summary of the Invention In general, the instant invention provides improved methods, systems and kits for rapid isolation of plasmid DNA from plasmid containing cells.

In one aspect, the invention features a method for the rapid isolation of plasmid DNA, including: a) collecting plasmid-containing cells and resuspending them in an aqueous buffer; b) incubating the resultant mixture with a lysis/denaturation solution to lyse the cells and denature DNA; c) neutralizing the mixture with a renaturation solution to generate a renatured mixture of dissolved plasmid DNA and flocculants containing insoluble genomic DNA and cellular debris; d) loading the renatured mixture directly to a modified spin column without first removing the flocculants from the mixture, which column having a pre-filtration disc (e.g., pre-filter) on top of a matrix; e) passing loaded sample mixture through the column such that the flocculants are packed on top of the pre- filtration disc while plasmid DNA binds to column matrix; f) washing the column with a wash solution to remove soluble impurities; and g) eluting plasmid DNA from the column with an elution buffer. It has been found surprisingly that by introducing an integral pre- filtration disc, there is no longer a need to remove flocculants containing cellular debris prior to loading the spin column. Instead, the flocculants stay on top of the pre-filtration

disc throughout the purification process and do not interfere with subsequent wash or elution of the plasmid DNA.

In a second aspect, the invention provides a modified spin column for the rapid isolation of plasmid DNA from plasmid-containing cells, comprising: a matrix; a support filter underneath the matrix; a pre-filter on top of the matrix; and a housing for the matrix and filters. Preferably, the modified spin column contains a glass fiber matrix and the pre- filter and support filters are made of porous sintered polyethylene. In a variation of the modified spin column, a depth filter is included between the separation matrix and the pre-filter to further enhance the performance of the system. In another aspect, the invention provides kits for rapidly isolating plasmid DNA, including the modification spin column, reagents and user manual.

Certain aspects of the invention allow simultaneous isolation of a large number of different plasmids. The spin modif columns can be joined together to take the form of a microtiter plate. By this kind of an arrangement, a number of different plasmid containing cell cultures can be processed simultaneously. It is noted that all centrifugation steps can be replaced with vacuum.

The above and further features and advantages of the instant invention will become clearer from the following detailed description and claims.

Brief Description of the Drawings

Figure 1 shows a schematic diagram of the spin modif column according to one embodiment of the invention.

Figure 2 shows a gel image of four samples prepared according to one example of the invention, before (left) and after (right) a HindIII digest. 400 ng of DNA was used for each digest, with 1 unit of HindIII, and incubated at 37⁰C for 2 hours. Far left: markers.

Figure 3 shows a schematic diagram of the modified spin column according to a variation of one embodiments of the invention. A depth filter is included between the pre-filter and the main separation matrix.

Figure 4 shows a gel image of plasmid DNA isolated using the modified combination pre- filter/depth filter systems according to the scheme of Figure 3. The numbers represent either controls or a particular combination according to Table 1.

Detailed Description of the Invention

The invention features improved processes, systems and kits for rapidly isolating plasmid DNA from plasmid containing cells, in particular for downstream applications in molecular biological research, such as cloning and sequencing. As used herein, the term "plasmid" refers to supercoiled DNA molecules (single or double stranded) that are maintained in a host cell separate from the host cell genome. Plasmids can be of a high copy number or low copy number and can carry any gene or external piece of DNA, either genomic or synthetic, encoding protein or peptide of interest, from any source.

In general, the improved process for isolating plasmid DNA includes modification of a spin column such that it eliminates the need to remove the insoluble flocculant cellular debris generated from the lysis of the cells, before loading the column, this simplifies the work flow and shortens the protocol significantly. A spin column for plasmid DNA isolation generally contains a separation matrix placed on and supported

physically by a disc of porous material more commonly referred to as a frit, typically made of sintered polyethylene. The holes so formed during the production of the frit material allow the unhindered passage of aqueous solutions and more importantly aqueous solutions containing plasmid DNA. The frit material is inert and does not interact to any great extent with DNA. The separation matrix is preferably a glass fiber matrix or a silica membrane. Alternatively, the matrix is a zeolite, or an organic matrix such as a resin or polymer.

One embodiment of the invention includes a modification of the spin column with the addition of an integral pre-filter on top of the separation matrix. One example of a pre-filter is a porous sintered polyethylene or polypropylene, similar to the support frit underneath the separation matrix.

The use of the pre-filtration disc does not have to be the same composition as the lower supporting frit and indeed an optimal column might be composed of alternative materials having different filtration/binding characteristics. A thinner "pre-filter" material (e.g., cellulose absorbent paper or polypropylene mesh) may allow improved assembly of the column where sheets of appropriate components are layered together prior to die- cutting and positioning within a column moulding. Optionally, an O-ring can be used to secure the "pre-filter" (Figure 1).

A variation of the embodiment additionally includes a depth filter between the pre-filter and the separation matrix (Figure 3). A combination of both a pre-filter and a depth filter further reduces residual contaminant flow-through from the pre-filter, thus is preferable for certain applications. A preferred depth filter is one that captures any residual flow-through contaminants from the pre-filter yet does not retain plasmid DNA during elution. A suitable depth filter is a glass microfiber filter.

Plasmids isolated in accordance with the invention can be of any origin. Most commonly, microorganisms like bacteria, such as Escherichia coli (E. coli), are used for culturing the plasmids, but the use of host cells is not limited and can be prokaryotic or eukaryotic cells. The host cells harboring the plasmid can be cultivated in a number of ways well known in the art, e.g. in incubator, bioreactor, fermentor etc. The plasmid isolated according to the invention can be of virtually any size, e.g. in the range of about 1 kb up to about 20 kb. As an upper limit, the isolation of cosmids and artificial chromosomes is also encompassed, the size of which may be up to about 50 kb and 500 kb, respectively. The modified spin column is suitable for extraction of plasmid DNA from standard cultures of bacteria. The inclusion of a pre-filter within a spin column eliminates the need to remove flocculant material generated by alkaline lysis, prior to addition of sample to the DNA-binding column. This modified column is especially suited for the so called miniprep of plasmid DNA from 1-5 ml overnight culture. For a miniprep, traditionally, lysate is clarified by a 5-10 minute spin in a micro-centrifuge before addition of the clarified lysate to the microspin column. Using the modified device, two steps are removed from the process without affecting quality of isolated product. Purification of plasmid DNA with the modified device can now be done in 6-8 minutes for a miniprep, compared to traditional process which typically takes about 20 minutes to complete.

The modified spin column is also suited for the preparation of plasmid DNA in a larger scale. For example, between 10-50 ml overnight culture could be used as a starting material, and larger spin columns are devised to accommodate the increased volume of the lysate. A modified, larger column with an integral prefilter achieves similar benefits as a modified microspin column.

During the experimentation it was found that the use of the pre-filter modified microspin column in combination with a fixed-angle microcentrifuge enabled the insoluble flocculent material to be pelleted "over to one side" so that occlusion of the frit pre-filter was less likely to occur. Even without a fixed-angle rotor, using a vacuum that distributes flocculant material across the entire surface of the frit, good quality plasmid DNA is still obtained that can be digested and sequenced.

By deploying the modified spin column, it has been possible to achieve multiple benefits. First, it enables the addition of lysed sample to the modified column without removal of flocculants (pre-processing). It also ensures total utilization of sample without incurring transfer losses owing to pre-processing. It further provides an improvement in the ease of use and time for completion, speeding up the process by more than 50%. The introduction of a pre-filter also stabilizes the separation matrix, known to be fragile and liable to partial fragmentation.

Methods for isolating plasmid DNA generally starts from culturing the host cells containing the plasmid. When the culture is ready, the cells are recovered by e.g. centrifugation or filtration. The cells can be stored, for example in a freezer, or processed immediately. The process for isolating plasmid DNA includes first collecting plasmid- containing cells and resuspending them in an aqueous buffer; then incubating with a lysis/denaturation solution to lyse the cells and denature DNA; followed by neutralizing the mixture with a renaturation solution to generate a renatured mixture of dissolved plasmid DNA and flocculants containing insoluble genomic DNA and cellular debris. In one aspect, the improved method includes loading the renatured solution with the flocculants directly to a modified spin column having an integral pre-filtration disc (pre- filter) on top of the separation matrix. The solution is then passed through the modified spin column by centrifugation or vacuum, such that the flocculants are packed on top of

the pre-filtration disc while plasmid DNA binds to separation matrix. The modified spin column is washed with a wash solution to remove soluble impurities; and plasmid DNA is eluted from the column with an elution buffer. It is surprisingly discovered that although the flocculants remain packed on top of the pre-filter during the washing and elution steps, high quality plasmid DNA is isolated that is suitable for subsequent molecular biology analysis.

The protocols for cell lysis and denaturation of cellular debris are well known. A particularly useful aqueous buffer for resuspending plasmid-containing cells contains an isotonic buffer (e.g. a Tris buffer; or a sucrose or glucose solution), a chelating agent (e.g. ethylenediaminetetraacetic acid (EDTA) or (CDTA)) and an RNAse. This buffer may also optionally include lysozyme to further weaken cell walls. After the cells are resuspended, the cells are lysed and linear DNA is denatured, preferably by incubation in an alkaline lysis solution. Thorough lysis and denaturation can be accomplished by mixing the resuspended cells with a sodium hydroxide, sodium dodecyl sulfate solution. A third, renaturation solution (e.g. an acetate buffered solution, containing a chaotropic salt) is then added to yield a mixture containing plasmid DNA, insoluble clots of linear DNA and cellular debris.

According to one aspect of the invention, the renatured mixture of dissolved plasmid DNA and insoluble flocculants are loaded to the modified spin column. Through vacuum or centrifugation, liquids in the mixture passes through the column, leaving on top of the pre-filter a packed layer of flocculants, in the meantime plasmid DNA binds to column matrix. A wash solution is then applied to remove soluble impurities; and plasmid DNA is then eluted from the modified spin column with an elution buffer. The flocculants remain packed on top of the pre-filtration disc during the washing and eluting steps but does not affect the quality of the plasmid DNA isolated.

The addition of a depth filter between the pre-filter and the separation matrix results in slightly better quality DNA. Thus it is preferable to include a depth filter in the modified spin column for certain preparations. The workflow, however, does not change from the protocol which includes the pre-filter only. Certain aspects of the invention allow simultaneous isolation of a large number of different plasmids. The modified spin columns can be joined together to take the form of a microtiter plate. Especially preferred are microtiter plates in the 96 well format. By this kind of an arrangement, a large number of plasmid containing cultures can be processed simultaneously. It is noted that all centrifugation steps can be replaced with vacuum.

Examples

The following examples serve to illustrate the plasmid DNA purification processes according to embodiments of the present invention and are not intended to be limiting.

1. The protocol

The protocol is suitable for the rapid extraction and purification of plasmid DNA from 1.5 ml cultures of E. coli. The procedure can be completed in less than 10 minutes to yield plasmid DNA with a purity and quality compatible with many common molecular biology techniques, including cloning, restriction enzyme digestion, PCR amplification and DNA sequencing.

The plasmid DNA yield from a freshly grown E. coli strain containing a high copy number plasmid (>300 copies/cell) and grown to A_60O approximately 2.5 is typically 4 to

The protocol utilizes a simple plasmid DNA purification process, employing a modified alkaline cell lysis procedure and a silica-based membrane. No organic solvents are used; instead, chaotropic salts are included to denature protein components and promote the selective binding of plasmid DNA to the silica membrane. Denatured insoluble contaminants are retained on top of pre-filter, while soluble contaminants are easily removed by subsequent washing. The purified plasmid DNA is eluted in a low ionic strength buffer, at a plasmid concentration suitable for most molecular biological applications.

The following provides a step by step protocol: 1. Transfer 1.5 ml from a fresh overnight culture to a microcentrifuge tube. To pellet bacteria, centrifuge (13 000 x g) for 30 seconds. Discard supernatant and re-centrifuge. Remove any residual supernatant using a pipette.

2. Thoroughly resuspend the pellet by adding 150 μl lysis buffer (10OmM Tris- HCl pH7.5; 1OmM EDTA; 0.2mg/ml RNase A), and either vortexing, pipetting up and down or scraping the base of the microcentrifuge tube across the surface of an empty pipette tip rack.

3. Cell lysis - Add 150 μl lysis buffer (20OmM NaOH; 1% SDS) and mix immediately by gentle inversion (approximately 5 times) until solution becomes clear and viscous. 4. Neutralisation - Add 300 μl neutralization buffer (4.4M Guanidine HCl,

0.65M potassium Acetate and 3.1M Glacial Acetic Acid), and mix immediately by gentle inversion until the precipitate is evenly dispersed. 5. Transfer the neutralized mixture to the modified microspin column

(approximately 600 μl). Close the lid of the column gently. Centrifuge (13

000 x g) for 30 seconds. Discard the flow through by emptying the collection tube.

6. Wash the column with 600 μl wash buffer (2mM Tris-HCl pH8; 0.2mM EDTA and 80% ethanol) and centrifuge (13 000 x g) for 30 seconds. Discard the flow-through and repeat the wash one more time with a 60 second spin.

7. Move the modified microspin column into a fresh microcentrifuge tube and add 100 μl elution buffer (1OmM Tris-HCl pH8) directly onto the centre of the column. Incubate the column for 30 seconds at room temperature. Microcentrifuge (2 000 x g) for 60 seconds to recover the plasmid DNA as flow through in the microcentrifuge tube.

Purified plasmid DNA concentration should be determined by UV spectrophotometry (A260) and through comparison with a known standard by agarose gel electrophoresis and subsequent densitometric analysis. If available, the UV spectrophotometric ratios A₂6o:A₂8o and A₂6o:A₂3o provide a limited indication of purity as measures of protein and salt contamination.

2. Purification of plasmid DNA using a modified microspin column containing a prefilter

Overnight cultures of E. coli TOPlO transformed with pCORON1002-EGFP-Cl were processed following the protocol described above. Four individual cultures were prepared and plasmid DNA was isolated according to the protocol. Modified microspin columns contained a pre-filtration disc of a porous, sintered polyethylene. The samples had a mean yield of 6.7 μg. The plasmids are suitable for downstream molecular biology applications as illustrated by restriction enzyme digestion (Figure T).

3. Purification of plasmid DNA using a variation of the modified microspin column

To further reduce extraction time whilst maintaining the purity/quality of the isolated DNA to a level comparable to that generated using traditional microspin systems, the inclusion of a depth column between the pre-fϊlter and the main separation matrix was tested (Figure 3).

The above protocol was used for plasmid DNA isolation, with slight modification. Briefly, 125 μl re-suspension buffer and lysis buffer, respectively, was used for each culture, while 250 μl neutralization buffer was used. Crude lysate was added directly onto the integral filtration/plasmid DNA binding column and centrifuged at 13,000 g for 60s in a microcentrifuge. The columns were washed twice with 400 μl wash buffer before DNA elution. Absorbance data was determined using a Nanodrop NDlOOO spectrophotometer.

A number of pre-filter and depth filter combinations were tested, using a silica membrane column as the main plasmid DNA binding matrix (the column from ILLUSTRA™ plasmidPrep Mini Spin kit). To compare the quality and yield with traditional protocols, controls were included. The control experiments were performed following manufacturer's protocols, except the pre-filter only control which was performed following the current protocol. The depth filter used was the Whatman GF/B glass microfibre depth filter. Table 1 lists the pre-filters tested in combination with the Whatman GF/B glass microfibre depth filter, and the control experiments performed.

Table 1 : Summary of pre-filter/depth filter combinations tested.

For each pre-fϊlter/depth filter combination (or control experiment), at least three parallel experiments were run. It was found that with an integral pre-filter/depth filter combination, the time needed to complete a plasmid DNA isolation experiment was about 7.5 min. In comparison, the ILLUSTRA™ plasmidPrep Mini Spin kit took about 9 min to complete, while the QIAPREP™ Spin Mini kit took about 19 min to complete. In general, the modified system with both a pre-filter and a depth filter generated comparable amount of plasmid DNA as the control extractions irrespective of the material used as the pre- filter.

The quality of the isolated plasmids were also comparable to the ones isolated using the control kits. Low level of protein contamination was observed. The amount of particulates in the final elution was also comparable to control extractions. Salt levels were lower than the control QIAPREP™ or ILLUSTRA™ plasmidPrep kit. The majority of native plasmid DNA was in the supercoiled configuration (Figure 4; 300 ng of DNA loaded on 1% agarose gel)). Therefore in general the quality of the isolated plasmid DNA was comparable to control extractions.

The modified microspin column with both a pre-filter and a depth filter combines the speed of a pre-filter only system (i.e. 7.5min) with quality associated with traditional spin extraction methods/kits. Even though the Whatman GF/B micro-fibre depth filter probably binds some plasmid DNA in the presence of the chaotrope, in an integral filter format the plasmid DNA can be recovered during the final elution step.

All patents, patent publications, and other published references mentioned herein are hereby incorporated by reference in their entireties as if each had been individually and specifically incorporated by reference herein. While preferred illustrative embodiments of the present invention are described, one skilled in the art will appreciate that the present invention can be practiced by other than the described embodiments, which are presented for purposes of illustration only and not by way of limitation. The present invention is limited only by the claims that follow.

Modification Raider 2010

SUZUKI RAIDER EXTREEME MODIFICATION

No more powerful features of the 2003 Suzuki Raider. Starting from the front to rear, this bike has changed completely. Instead the result so extreme, with some elements of the technology changes. Like condoms tank lid can be opened using keys, that one is a teroboson. At the very least, a breakthrough modifications in Pontianak, West Kalimantan.

Home modifications Irfandi from Pontianak in 2XP "Equator City" made a bold move. Consider the attached seat on the house from the original exhaust Ducati 969. Meanwhile, the bottom-distance with the wheels still empty. "Indeed, the design was not able to fit," said Irfandi.

Plus the arm (swing arm) are large and sturdy, while the framework for and the seat is thin, the harmonization was a little disturbed. "In the arm, I use the pro arm of Honda NSR SP and condoms to be given greater," explained Irfandi.

To the top, using a subframe which modifikator is knock down because he wanted to make a long tail. If you get bored, then it could be dismantled extra-pair. To integrate additional context, he uses the L bolt size of 8 mm 6 pieces. "In addition to booster seats, it also became a kind of bracket for the exhaust," said senior modifikator.

SUZUKI RAIDER EXTREEME MODIFY

Realized there was a wide cavity, he cut back by adding exhaust Ducati 969. Another thing if a minimoto sokbreker position in Australia was not too collapsed. For each, he wore a Honda CBR150 for more tender than the original.

Also interesting, given condoms tank-lid can be opened by pressing the button. According to Irfandi, he uses the power window car fitted with a small shock. By pressing once, the condom could be opened up to 45 degrees.

SUZUKI RAIDER EXTREEME MODIFICATION

SUZUKI RAIDER EXTREEME MODIFIED

BLUE SUZUKI SATRIA FU 2010 FULL MODIFICATION

SUZUKI SATRIA FU 2010 FULL MODIFICATION WALLPAPER

Although we intip ahead, but who knows if in actuality there are several new appearance looks abaft the New Satria F150. “There are some appearance that differ,” Edi bisik begin in the average ramainya JMS 2008.Dari on let’s accessible it’s the latest detail avoid with agent accommodation of at best this.

Most accessible change would accept on the architecture of the lamp cover. Head lamp architecture and covernya become the capital attraction. Suzuki already has from the aboriginal appropriate of MOGE consistently accommodate a blow of avoid including the motor for this latest Satria F150. Head lamp Suzuki GSX 600R entered to try to abbreviate the anatomy Suzuki SATRIA F150.

SUZUKI SATRIA FU 2010 FULL MODIFICATION

Great! it is the appropriate chat accustomed to the Suzuki Satria F-150 this. Imagine, Topo Goedhel Atmodjo’s Custom Tauco (TC), so alteration the appearance of a avoid action archetypal with the anatomy allotment is minimal. “With the anatomy allotment that can additionally appearance little adeptness to comedy detail,” said Topo.

SUZUKI SATRIA FU 2010 FULL MODIFICATION PICTURES

As the adjustment of Tubular adamant pipe, for example. Usually, in the average allotment is larboard empty, but bankrupt by Topo use triangular plate. Not accessible assignment because the bankrupt charge bethink the absolute contours of anniversary acreage have.

Suzuki show off their new 'G-Strider'

G-Strider, not G-String, is the name given to a new vision that Suzuki recently revealed at the Tokyo Motor Show. The ergonomic feet-forward design approach is complemented by an unprecedented level of information technology and electronic assistance on a motorcycle. "Suzuki's G-strider is all about pushing the boundaries, introducing new ideas and speculating on where motorcycle design might go in the future," Perry Morison, General Manager - Motorcycles, Suzuki Australia, said. "As with previous concepts such as the Hayabusa-powered B-King, Suzuki has incorporated technology in the G-Strider that may be pure fantasy or may make production next year ... that's what makes the concept so interesting." Powered by a 916cc, four-stroke, liquid-cooled, DOHC, parallel twin engine, the G-Strider utilises Suzuki's Electronically-controlled Continuously Variable Transmission (SECVT), as found on the Suzuki Burgman 650 Superscooter. Electronics feature extensively in the Suzuki G-Strider's design, with electrically adjustable handlebars, windscreen, rider's seat, footrests, and backrests for both rider and passenger, enabling selection of the most comfortable seating position possible. The Suzuki G-Strider also features an advanced telematics system which utilises a bidirectional wireless infrastructure to support videophone communication and Global Positioning System (GPS) navigation assistance. The G-Strider is equipped with an intelligent keyless ignition system and a self-diagnosis function, in which data from a range of sensors is analysed and results displayed on the liquid-crystal main monitor located behind the windscreen. In addition, a handlebar-mounted digital meter with electroluminescent backlighting provides easily readable information about the bike's key functions. A unique tank-mounted console with trackball control and large function keys, which can be easily operated by gloved hands. Futuristic safety features on the Suzuki G-Strider include two rear-facing cameras mounted in the rear lighting system, which feed video images to the rear view monitors, replacing conventional mirrors. A high-intensity-discharge projector-type light integrated with high-intensity light-emitting diodes (LEDs) form a powerful combination headlight, while the tail and indicator lights also employ high intensity LEDs, making the G-Strider more visible to surrounding traffic, even in broad daylight. An intelligent cornering lamp system, which uses microprocessors to sense the motorcycle's bank and handlebar angle, illuminates the road on the inside of the turn accordingly. Suzuki's G-Strider also incorporates a centre-hub steering system and a compound-laser welded front swingarm for enhanced rider control, and an anti-lock brake system (ABS), with the front brakes using radial-mount four-piston calipers. The package is mounted on impressive 140mm front and massive 220mm diameter rear tyres to further its handling and stability. Suzuki G-Strider - Specifications Engine type: Liquid cooled, four-stroke, DOHC, four valves per cylinder, parallel-twin cylinder engine Displacement: 916cc Transmission: Suzuki Electronically-controlled Continuously Variable Transmission (SECVT) Overall length: 2,445mm Overall width: 710mm Overall height: 1,170mm Wheelbase: 1,800mm Seat height: 615mm Tyres: 140/60R17 (F) - 220/40R18 (R)